Performance Specification of the Laboratories for doping control
required by the International Federation of Horseracing Authorities
(cf. to Article 6 A § 19: http://www.ifhaonline.org/default.asp?section=IABRW&area=2#article6a)
This specification describes the minimum analytical capability required to detect exposure. It must not be construed as restricting in any way the chemical groups or concentrations that may constitute positive findings. Many laboratories will be able to surpass this specification, and the interpretation of whether a finding and a particular concentration would constitute a violation is governed by the relevant Rules of Racing.
Procedures should ensure that the laboratory can find prohibited substances in equine body fluids and prove their presence. Test methods must cover the chemical groups typified by the representative list. Each substance in the representative list must be detectable and identifiable at the concentration shown, if necessary after hydrolysis of metabolic conjugates. In addition, test methods must also reliably quantify threshold substances at concentrations that would be declared positive.
Note 1: These concentrations must not be construed as regulatory thresholds or screening limits.Representative substance | Minimum concentration (ng/mL in horse urine) for detecting exposure |
2-(1-Hydroxyethyl)promazine sulfoxide | 10 |
16β-Hydroxy-stanozolol | 1 |
Amitriptyline | 50 |
Amphetamine | 10 |
Atenolol | 10 |
Benzoylecgonine | 20 |
Betamethasone | 0.2 |
Boldenone or Boldenone sulfate (analyte) | 40 ng/mL Boldenone sulfate (in horse urine) |
Bumetanide | 10 |
Butorphanol | 5 |
Caffeine | 100 |
Chlorpromazine | 20 |
Clenbuterol | 2 |
Dermorphin | 10 |
Detomidine, 3'-hydroxy | 5 |
Dexamethasone | 0.2 |
Ephedrine | 20 |
Epitrenbolone (17α-Trenbolone) | 1 |
Ethacrynic acid | 100 |
Firocoxib | 20 |
Fludrocortisone | 10 |
Flufenamic acid | 50 |
Flumethasone | 20 |
Flunixin | 50 |
Fluoxetine | 20 |
Fluphenazine | 5 |
Frusemide | 100 |
Glycopyrrolate | 2 |
Guanabenz | 10 |
Hydrochlorothiazide | 200 |
Hydromorphone | 2 |
Ibuprofen | 100 |
Ipratropium | 1 |
Ketoprofen | 100 |
Ketorolac | 100 |
Lidocaine, 3'-hydroxy | 25 |
Meclofenamic Acid | 100 |
Meloxicam | 25 |
Mephentermine | 10 |
Mepivacaine, 3’-hydroxy | 10 |
Methocarbamol | 200 |
Morphine or Morphine-3-glucuronide (analyte) | 50 ng/mL Morphine-3-glucuronide (in horse urine) |
Nikethamide | 100 |
Nordazepam | 20 |
Oxazepam | 20 |
Oxymorphone | 2 |
Pethidine (Meperidine) | 10 |
Phenazone (Antipyrine) | 300 |
Phenobarbitone (Phenobarbital) | 50 |
Pseudoephedrine | 20 |
Ractopamine | 5 |
Ritalinic acid | 20 |
Salbutamol (Albuterol) | 2 |
Sildenafil | 5 |
Tenoxicam | 20 |
Terbutaline | 10 |
Tramadol, O-desmethyl | 5 |
Triamcinolone acetonide | 2 |
Venlafaxine, O-desmethyl | 5 |
Xylazine, 4'-hydroxy | 10 |
Representative substance | Minimum concentration (ng/mL in horse plasma) for detecting exposure |
Acepromazine | 1 |
Amitriptyline | 1 |
Atenolol | 1 |
Benzoylecgonine | 2 |
Betamethasone | 0.2 |
Boldenone | 0.1 |
Butorphanol | 1 |
Caffeine | 10 |
Chlorpromazine | 1 |
Clenbuterol | 1 |
Codeine | 10 |
Detomidine | 0.1 |
Dexamethasone | 0.2 |
Diazepam | 10 |
Diclofenac | 5 |
Ephedrine | 10 |
Firocoxib | 20 |
Flufenamic acid | 10 |
Flumethasone | 1 |
Flunixin | 20 |
Fluphenazine | 0.2 |
Ketamine | 1 |
Ketoprofen | 10 |
Lidocaine | 1 |
Meloxicam | 5 |
Mepivacaine | 0.1 |
Methocarbamol | 1 |
Methylphenidate | 1 |
Methylprednisolone | 0.1 |
Nandrolone | 1 |
Nordazepam | 1 |
Pethidine (Meperidine) | 20 |
Pentazocine | 1 |
Piroxicam | 5 |
Pseudoephedrine | 10 |
Prednisolone | 5 |
Reserpine | 1 |
Salbutamol (Albuterol) | 1 |
Sildenafil | 1 |
Stanozolol | 1 |
Terbutaline | 5 |
Testosterone | 0.5 |
Tramadol | 1 |
Triamcinolone acetonide | 0.1 |
Xylazine | 0.1 |